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Variabilnost nukleotidnih zaporedij dela kontrolne regije mitohondrije DNA pripostrveh Salmo trutta : diplomska naloga = DNA sequence variability of the mitochondrial control region in Brown trout Salmo trutta : graduation thesisMelkič, EnverPreučevali smo variabilnost nukleotidnega zaporedja dela kontrolne regije mitohondrijske DNA (mtDNA) pri populacijah potočne postrvi (Salmo trutta f. fari) atlantskega in donavskega tipa ter pri ... soški postrvi (Salmo marmoratus). Mitohondrijsko DNA smo izolirali iz krvi ter željene fragmente dela kontrolne regije mtDNA pomnožili z verižno reakcijo s polimerazo. Variabilnost nukleotidnega zaporedja dela kontrolne regije mtDNA smo ugotavljali z restrikcijsko analizo, elektroforezo enojnih verig na poliakril amidnem gelu (PCR-SSCP) in s sekvenciranjem. Na DNA fragmentu prvega dela kontrolne regije mtDNA (426 bp) smo našli nukleotidne variante, na osnovi katerih smo lahko zanesljivo ločili atlantski tip potočne postrvi (haplotip C)od donavskega (haplotipa A in B) in od soškega tipa postrvi (haplotip D). Znotraj donavskega tipa potočne postrvi smo odkrili dve novi genetski varianti, ki označujeta haplotipa BA in BKr, ki pripadata donavskemu tipu potočnih postrvi in sta značilni za slovenske reke donavskega porečja. Na osnovi genetskih razlik smo ovrednotili filogenetske odnose med navedenimi tipi postrvi.Type of material - undergraduate thesisPublication and manufacture - Domžale : [E. Melkič], 2000Language - slovenianCOBISS.SI-ID - 1122440
Author
Melkič, Enver
Other authors
Dovč, Peter, 1958- |
Snoj, Aleš
Topics
molekularna biologija |
molekularna genetika |
ribe |
Salmo marmoratus |
soška postrv |
Salmo trutta |
potočna postrv |
polimorfizem DNK |
mitohondrijska DNK |
genetski markerji |
diplomske naloge
| Library/institution |
City | Acronym | For loan | Other holdings |
|---|---|---|---|---|
| Biotechnical Faculty, Ljubljana | Ljubljana | BFLJ |
reading room 1 cop.
|
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DRS, in which the journal is indexed
| Database name | Field | Year |
|---|
| Links to authors' personal bibliographies | Links to information on researchers in the SICRIS system |
|---|---|
| Dovč, Peter, 1958- | 05098 |
| Snoj, Aleš | 11906 |
Source: Personal bibliographies
and: SICRIS
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