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Preliminary analysis of AZFb region duplication by quantitative real-time PCR
Writzl, Karin ; Zorn, Branko ; Peterlin, Borut, 1963-
Background: Deletions of the AZFb region on the long arm of the human Y chromosome cause male infertility. However, the reciprocal products of these deletion events, AZFb duplications, have not been ...reported to date. Furthermore, it is not known whether potential AZFb duplications represent a risk factor for spermatogenic failure. Methods: A total of 150 patients with male idiopathic subfertility (79 non-obstructive azoospermics and 71 oligozoospermics) and 150 fertile men were analysed for deletion/duplication of the sY125 locus and of the JARIDID gene using real-time PCR. Results: Threeazoospermic men had deletion of the sY125 locus and of the JARIDID gene. No duplication was detected. Conclusions: In our limited sample, AZFb duplications do not appear to be associated with male infertility.
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