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  • Uropathogenic escherichia coli induces serum amyloid A in mice following urinary tract and systemic inoculation [Elektronski vir]
    Erman, Andreja ...
    Serum amyloid A (SAA) is an acute phase protein involved in the homeostasis ofinflammatory responses and appears to be a vital host defense component withprotective anti-infective properties. SAA ... expression remains poorly defined in many tissues, including the urinary tract which often faces bacterial challenge. Urinary tract infections (UTIs) are usually caused by strains of uropathogenic Escherichia coli (UPEC) and frequently occur among otherwise healthy individuals, many of whom experience bouts of recurrent and relapsing infections despite the use of antibiotics. To date, whether SAA is present in the infected urothelium and whether or not the induction of SAA canprotect the host against UPEC is unclear. Here we show, using mouse models coupled with immunofluorescence microscopy and quantitative RT-PCR, that delivery of UPEC either directly into the urinary tract via catheterization orsystemically via intraperitoneal injection triggers the expression of SAA. As measured by ELISA, serum levels of SAA1/2 were also transiently elevated inresponse to UTI, but circulating SAA3 levels were only up-regulated substantially following intraperitoneal inoculation of UPEC. In in vitro assays, physiological relevant levels of SAA1/2 did not affect the growth or viability of UPEC, but were able to block biofilm formation by the uropathogens. We suggest that SAA functions as a critical host defense againstUTIs, preventing the formation of biofilms both upon and within the urothelium and possibly providing clinicians with a sensitive serological marker for UTI.
    Source: PloS one [Elektronski vir]. - ISSN 1932-6203 (Vol. 7, iss. 3, Mar. 2012, str. [1-7], e32933)
    Type of material - e-article
    Publish date - 2012
    Language - english
    COBISS.SI-ID - 29697497

    Link(s):

    http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0032933
    DOI

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