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  • Quantification of (-)-Epicatechin and Procyanidin B2 in Chocolates
    Glavnik, Vesna, 1980- ...
    Validation of a method for the quantification of (-)-epicatechin and procyanidin B2 in chocolates is reported. After a simple preparation of the chocolate test solution, thin-layer chromatography ... (TLC) on high-performance thin-layer chromatographic (HPTLC) cellulose plates with n-propanol-water-acetic acid (20:80:1, v/v) and derivatization with 4-dimethylaminocinnamaldehyde (DMACA) were performed. Densitometry at 655 nm enabled accurate quantification of both analytes in chocolates. The milk chocolate sample which served for precision and recovery experiments contained13.0 mg/100 g of (-)-epicatechin (relative standard deviation (RSD)) 5.8%, n = 6) and 13.2 mg/100 g of procyanidin B2 (RSD 4.2%, n = 6). Usable polynomial curves were 2-30 ng for (-)-epicatechin and 4-60 ng for procyanidinB2. The validated TLC method can be applied for routine quality control of the two major polyphenolic compounds in different chocolate samples.
    Source: JPC. Journal of planar chromatography, modern TLC. - ISSN 0933-4173 (Vol. 24, no. 6, 2011, str. 482-486)
    Type of material - article, component part
    Publish date - 2011
    Language - english
    COBISS.SI-ID - 4854554

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