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  • Unperturbed islet ▫$\alpha$▫-cell function examined in mouse pancreas tissue slices [Elektronski vir]
    Huang, Ya-Chi ; Rupnik, Marjan ; Gaisano, Herbert Y
    Critical investigation into alpha-cell biology in health and diabetes has beensparse and at times inconsistent because of the technical difficulties with employing conventional strategies of isolated ... islets and dispersed singlecells. An acute pancreas slice preparation was developed to overcome theenzymatic and mechanical perturbations inherent in conventional islet cell isolation procedures. This preparation preserves intra-islet cellular communication and islet architecture in their in situ native state. Alpha-cells within tissue slices are directly assessed by patch pipette and electrophysiologically characterized. The identity of the patched cells was confirmed by biocytin dye labeling and immunocytochemistry. Alpha-cells in mouse pancreas slices exhibited well-described features of INa (excitable at physiological membrane potential), IKATP, small cell size, low resting membrane conductance, and inducible low and high voltage-activated ICa, the latter correlating with exocytosis determined by capacitance measurements. In contrast to previous reports, our large unbiased sampling of alpha-cells revealed a wide range distribution of all of these parameters, including the amount of KATP conductance, Na+ and Ca2+ current amplitudes, and capacitance changes induced by a train of depolarization pulses. The proposed pancreas slice preparation in combination with standard patch-clamping technique allowed large sampling and rapid assessment of alpha-cells, which revealed a wide distribution in alpha-cell ion channel properties. This specific feature explains the apparent inconsistency of previous reports on these alpha-cell ion channel properties. Our innovative approach will enable future studies into elucidating islet alpha-cell dysregulation occurring during diabetes.
    Source: The journal of physiology. - ISSN 0022-3751 (15. jan. 2011, str. 395-408)
    Type of material - e-article ; adult, serious
    Publish date - 2010
    Language - english
    COBISS.SI-ID - 512107576
    DOI

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