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Synthesis of estrogens in cell lines of endometrial and ovarian cancer : M. Sc. Thesis : Master Study Programmes = Sinteza estrogenov v celičnih linijah raka endometrija in jajčnikov : magistrsko delo : magistrski študij - 2. stopnja
Gjorgoska, Marija
Endometrial (EC) and ovarian (OC) cancers are generally considered as post-menopausal, hormone-dependent pathologies with increasing incidence and mortality rate. Estrogen intracrine action is ...regarded vital in the cancer initiation and progression. Estrogens can form locally from androgen and estrogen precursors, through the aromatase and the sulfatase pathway, respectively. Here we intend to elucidate the contribution of the sulfatase pathway to active estrogen formation in model cell lines of EC and OC, by means of liquid chromatography-tandem mass spectrometry (LC-MS/MS). For this purpose we developed a robust LC-MS/MS method with a picomolar sensitivity for the following analytes: estrone (E1), estrone sulfate (E1-S), estradiol (E2), and estradiol sulfate (E2-S). Control and mode l cell lines of EC andOC were incubated with different E1-S concentrations for 8, 24, 48, and 72 hours, and the formed metabolites analyzed by LC-MS/MS. Our data indicate that the sulfatase pathway contributes to the estrogen formation actively, yet differently in EC and OC model cell lines and the respective control lines. More specifically, greater levels of E1and E2 are synthesized in the EC and OC model cell lines comparing to the respective control lines, altogether implyingan active role of estrogen in EC and OC carcinogenesis, acting as pro-proliferative hormones and/or genotoxic agents.
Type of material - master's thesis
; adult, serious
Publication and manufacture - Ljubljana : [M. Gjorgoska], 2021
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