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  • Flow cytometry of HEK 293T cells interacting with polyelectrolyte multilayer capsules containing fluorescein-labeled poly(acrylic acid) as a pH sensor
    Reibetanz, Uta ...
    Polyelectrolyte multilayer sensor capsules, 5 m in diameter, which contained fluorescein-labeled poly(acrylic acid) (PAAAF) as pH-sensitive reporter molecules, were fabricated and employed to explore ... their endocytotic uptake into HEK 293T cells by flow cytometry. The percentage of capsules residing in the endolysosomal compartment was estimated from the fluorescence intensity decrease caused by acidification. Capsules attached to the extracellular surface of the plasma membrane were identified by trypan blue quenching. The number of capsules in the cytoplasm was rather small, being below the detection limit of the method. The advantages of polyelectrolyte multilayer capsules are that the fluorophore is protected from interaction with cellular compartments and that the multilayer can be equipped with additional functions.
    Vir: Biomacromolecules. - ISSN 1525-7797 (Vol. 8, iss. 6, 2007, str. 1927-1933)
    Vrsta gradiva - članek, sestavni del ; neleposlovje za odrasle
    Leto - 2007
    Jezik - angleški
    COBISS.SI-ID - 15383304

    Povezava(-e):

    http://www.hubmed.org/display.cgi?uids=17508712

vir: Biomacromolecules. - ISSN 1525-7797 (Vol. 8, iss. 6, 2007, str. 1927-1933)
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