VSE knjižnice (vzajemna bibliografsko-kataložna baza podatkov COBIB.SI)
  • Expression of proteins using the third domain of the Escherichia coli periplasmic-protein TolA as a fusion partner
    Anderluh, Gregor, 1969- ; Gökce, Isa ; Lakey, Jeremy H.
    The third domain of the periplasmic protein To1A from Escherichia coli (To1AIII) was used as a fusion partner in the expression of various proteins from bacteria and eukaryotes. To1AIII is small ... domain, expressed in high yields as a soluble protein in the cytoplasm of E. coli. Proteins were linked to the C-terminus of To1AIII by a short flexible linker containing sites for endopeptidases. Three different vectors were prepared, containing sites for enterokinase, thrombin or factor Xa. Fusion proteins also contain a His6Ser2 tag at their N-terminus for easier purification. Up to 90 mg fusion protein per liter bacterial culture was obtained using these vectors. Colicin N R-domain was expressed with this system as a fusion and processed further for functional studies. The yield of final pure R-domain was doubled as compared to the direct expression. The system may prove to be useful in the preparationof other peptides and proteins.
    Vir: Protein expression and purification. - ISSN 1046-5928 (Letn. 28, 2003, str. 173-181)
    Vrsta gradiva - članek, sestavni del
    Leto - 2003
    Jezik - angleški
    COBISS.SI-ID - 16160729

Vnos na polico