VSE knjižnice (vzajemna bibliografsko-kataložna baza podatkov COBIB.SI)
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Exploring the safety of cannabidiol (CBD) [Elektronski vir] : a comprehensive in vitro evaluation of the genotoxic and mutagenic potential of a CBD isolate and extract from Cannabis sativa LŠtern, Alja ...Cannabidiol (CBD), a naturally occurring cyclic terpenoid found in Cannabis sativa L., is renowned for its diverse pharmacological benefits. Marketed as a remedy for various health issues, CBD ... products are utilized by patients as a supplementary therapy or post-treatment failure, as well as by healthy individuals seeking promised advantages. Despite its widespread use, information regarding potential adverse effects, especially genotoxic properties, is limited. The present study is focused on the mutagenic and genotoxic activity of a CBD isolate (99.4 % CBD content) and CBD-rich Cannabis sativa L extract (63.6 % CBD content) in vitro. Both CBD samples were non-mutagenic, as determined by the AMES test (OECD 471) but exhibited cytotoxicity for HepG2 cells (~IC50 (4 h) 26 µg/ml, ~IC50 (24 h) 6–8 µg/ml, MTT assay). Noncytotoxic concentrations induced upregulation of genes encoding metabolic enzymes involved in CBD metabolism, and CBD oxidative as well as glucuronide metabolites were found in cell culture media, demonstrating the ability of HepG2 cells to metabolize CBD. In this study, the CBD samples were found non-genotoxic. No DNA damage was observed with the comet assay, and no influence on genomic instability was observed with the cytokinesis block micronucleus and the γH2AX and p-H3 assays. Furthermore, no changes in the expression of genes involved in genotoxic stress response were detected in the toxicogenomic analysis, after 4 and 24 h of exposure. Our comprehensive study contributes valuable insights into CBD’s safety profile, paving the way for further exploration of CBD’s therapeutic applications and potential adverse effects.Vir: Biomedicine & pharmacotherapy [Elektronski vir]. - ISSN 1950-6007 (Vol. 177, [article no.] 116969, Aug. 2024, str. 1-13)Vrsta gradiva - e-članek ; neleposlovje za odrasleLeto - 2024Jezik - angleškiCOBISS.SI-ID - 200108803
Digitalne vsebine: dCOBISS
| Avtor | Štern, Alja ... |
| Naslov | Exploring the safety of cannabidiol (CBD) [Elektronski vir] : a comprehensive in vitro evaluation of the genotoxic and mutagenic potential of a CBD isolate and extract from Cannabis sativa L |
| Datum objave | 2024-06-21 |
| COBISS.SI-ID | 200108803 |
| Verzija objave v repozitoriju | Založnikova različica |
| Licenca objave v repozitoriju | Creative Commons Priznanje avtorstva-Nekomercialno 4.0 Mednarodna |
| Embargo | Takojšnja javna objava |
Projekti in financerji, navedeni v objavi
| Naziv | Akronim | Številka projekta | Financer |
|---|---|---|---|
| Ekotoksiologija, toksikološka genomika in karcinogeneza | P1-0245-2019 |
Javna agencija za znanstvenoraziskovalno in inovacijsko dejavnost Republike Slovenije |
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| Vrednotenje varnosti kanabidiolov in pomen za javno zdravje in vedenje potrošnikov | L3-3177-2021 |
Javna agencija za znanstvenoraziskovalno in inovacijsko dejavnost Republike Slovenije |
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| TWINNING FOR EXCELLENCE TO STRATEGICALLY ADVANCE RESEARCH IN CARCINOGENESIS AND CANCER | CutCancer | 101079113 |
European Commission |
Datoteke, ki spadajo k objavi
https://www.sciencedirect.com/science/article/pii/S0753332224008539
Stroški objave
| Vrsta stroška | Skupni stroški | Plačila |
|---|---|---|
| APC – strošek objave članka | 3733.20 € |
Vir: Osebne bibliografije
in: SICRIS
