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  • A multifaceted approach to understanding protein-buffer interactions in biopharmaceuticals
    Lebar, Blaž ...
    The excipient selection process plays a crucial role in biopharmaceutical formulation development to ensure the long-term stability of the drug product. Though there are numerous options approved by ... regulatory authorities, only a subset is commonly utilized. Previous research has proposed various stabilization mechanisms, including protein-excipient interactions. However, identifying these interactions remains challenging due to their weak and transient nature. In this study, we present a comprehensive approach to identify such interactions. Using the 1HT2 CPMG (Carr-Purcel-Meiboom-Gill) filter experiment we identified interactions of rituximab with certain buffers and amino acids, shedding light on its Fc fragment instability that manifested during the enzymatic cleavage of the antibody. Moreover, chemometric analyses of 2D NMR fingerprints revealed interactions of selected excipients with antibody fragments. Furthermore, molecular dynamics simulations revealed potential interacting hotspots without NMR spectra assignment. Our results highlight the importance of an orthogonal methods approach to uncovering these critical interactions, advancing our understanding of excipient stabilization mechanisms and rational formulation design in biopharmaceutics.
    Vir: European journal of pharmaceutics and biopharmaceutics. - ISSN 0939-6411 (Vol. 206, 2025, 12 str.)
    Vrsta gradiva - članek, sestavni del ; neleposlovje za odrasle
    Leto - 2025
    Jezik - angleški
    COBISS.SI-ID - 216273923

    Povezava(-e):

    https://www.sciencedirect.com/science/article/pii/S0939641124004089
    Repozitorij Univerze v Ljubljani – RUL
    DOI

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