VSE knjižnice (vzajemna bibliografsko-kataložna baza podatkov COBIB.SI)
  • Vpletenost endotelija pri učinkih ekvinatoksina II na izolirano prašičjo koronarno arterijo
    Kirbiš, Simona, 1976-
    Background: Equinatoxin II (EqT II) is a lethal basic protein isolated from the sea anemone Actinia equina (L.) and acts as actinoporin. The putative cause of death in experimental animals is ... cardiorespiratory arrest. The principal reason of the cardiotoxic effects of the toxin and the mechanism of reduction of coronary flow after the application of EqT II is poorly understood. Objective and hypothesisČ In the present study we investigated theeffects of EqT II on the isolated porcine left anterior descending coronaryartery. We tried to confirm the hypothesisČ 1) EqT II releases from endothelial cells endothelin-1 (ET-1) that mediates vasoconstriction, and 2) Tezosentan, an experimental nonspecific ETA and ETB (both are ET-1 receptors) endothelin receptor antagonist, competitively antagonises effects of ET-1 alsoin porcine coronary artery. Methods: Contractions were measured with mechanical-electrical transducer. We compared the contraction induced by EqT II with the contraction of 30 mM KCl in the same isolated ring of coronary artery and expressed it as the relative value. Results were expressed as mean +- standard error of the mean. ResultsČ Equinatoxin II dose dependently contracted isolated coronary artery with intact endothelium. In arteries with removed endothelium EqT II did not cause measurable contractions of the isolated artery. Addition of the endothelin receptor antagonist tezosentan in 1 micro M concentration reduced the contraction of porcine coronary artery induced by EqT II for more than 60%. Conclusions: As in our experiments the endothelin receptor antagonist tezosentan significantly reduced the contraction of porcine coronary artery induced by EqT II, these results allow the conclusion that ET-1 mediates the vasoconstriction induced by EqT II.
    Vrsta gradiva - raziskovalno poročilo
    Založništvo in izdelava - Ljubljana : [S. Kirbiš], 2000
    Jezik - slovenski
    COBISS.SI-ID - 2266132

Knjižnica/institucija Kraj Akronim Za izposojo Druga zaloga
MF, Centralna medicinska knjižnica, Ljubljana Ljubljana CMK na dom 1 izv.
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