VSE knjižnice (vzajemna bibliografsko-kataložna baza podatkov COBIB.SI)
  • Novel nucleus-oriented quenched activity-based probes link cathepsin nuclear localization with mitosis [Elektronski vir]
    Reut Shannon, Karin ...
    Cysteine cathepsins are important proteases that are highly upregulated in cancers and other diseases. While their reported location is mostly endolysosomal, some evidence shows their nuclear ... localization and involvement in the cell cycle. We aim to generate tools to investigate the involvement of cathepsins in the cell cycle progression. To investigate nuclear cathepsin activity, we designed nucleus-directed quenched activity-based probes (qABPs) by attaching cell-penetrating peptides (CPPs). qABPs are active-site-directed compounds that enable direct real-time monitoring of enzyme activity by the covalent linkage between the probe and the enzyme’s active site. Biochemical evaluation of the CPP-qABPs showed potent and selective probes; cell fractionation, multimodal flow cytometry-imaging, and time-lapse movies demonstrated nuclear cathepsin activity in living cells. Interestingly, these probes reveal a spatiotemporal pattern, a surge of nuclear cathepsin just before mitosis, suggesting yet unrevealed roles of cathepsin in cell division. In summary, these nuclear-directed qABPs serve as unique scientific tools to unlock the hidden features of cysteine proteases and to understand their involvement in cell division and cancer.
    Vir: ACS sensors. - ISSN 2379-3694 (Vol. 10, iss. 2, Feb. 2025, str. 1321-1333)
    Vrsta gradiva - e-članek ; neleposlovje za odrasle
    Leto - 2025
    Jezik - angleški
    COBISS.SI-ID - 259960835

    Povezava(-e):

    https://pubs.acs.org/doi/10.1021/acssensors.4c03217
    DOI

Vnos na polico