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  • Alteration of antibody specificity during isolation and storage
    Omersel, Jasna ...
    Isolation buffer characteristics and storage conditions could partially transform natural antibodies. 50 IgG factions were isolated from seven healthydonors' sera using various protein G columns and ... buffers. PAGE revealedno major antibody cleavages; purity of IgGs eluted at pH 2.4-3.5 was close to homogeneity, independent of buffer composition. Although eluting at pH 2.4 resulted in 3.5- or 17-fold higher antibody yield compared to pH 3.0 or3.5, respectively, it distorted the antibody molecule. IgGs eluted at pH 2.4acquired reactivity against diagnostically important autoantigens, confirmed by standardized ELISAs. Preserved antibodies' natural activity is important in further experiments with oxidatively-induced autoantibodies.
    Vir: Journal of immunoassay & immunochemistry. - ISSN 1532-1819 (no. 1, Vol. 31, 2010, str. 45-59)
    Vrsta gradiva - članek, sestavni del
    Leto - 2010
    Jezik - angleški
    COBISS.SI-ID - 26457305

    Povezava(-e):

    http://www.informaworld.com/smpp/content~db=all~content=a918160129
    DOI

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