VSE knjižnice (vzajemna bibliografsko-kataložna baza podatkov COBIB.SI)
  • Serum starvation : caveat emptor
    Pirkmajer, Sergej ; Chibalin, Alexander V.
    Serum starvation is one of the most frequently performed procedures in molecular biology and there are literally thousands of research papers reporting its use. In fact, this method has become so ... ingrained in certain areas of research that reports often simply state that cells were serum starved without providing any factual details as to how the procedure was carried out. Even so, we quite obviously lack unequivocal terminology, standard protocols, and perhaps most surprisingly, a common conceptual basis when performing serum starvation. Such inconsistencies not only hinder interstudy comparability but can lead to opposing and inconsistent experimental results. Although it is frequently assumed that serum starvation reduces basal activity of cells, available experimental data do not entirely support this notion. To address this important issue, we studied primary humanmyotubes, rat L6 myotubes and human embryonic kidney (HEK)293 cells underdifferent serum starvation conditions and followed time-dependent changesin important signaling pathways such as the extracellular signal-regulated kinase 1/2, the AMP-activated protein kinase, and the mammalian target of rapamycin. Serum starvation induced a swift and dynamic response, which displayed obvious qualitative and quantitative differences across different cell types and experimental conditions despite certain unifying features. There was no uniform reduction in basal signaling activity.Serum starvation clearly represents a major event that triggers a plethora of divergent responses and has therefore great potential to interferewith the experimental results and affect subsequent conclusions.
    Vir: American journal of physiology. cell physiology. - ISSN 0363-6143 (Vol. 301, issue 2, 2011, str. C272-C279)
    Vrsta gradiva - članek, sestavni del
    Leto - 2011
    Jezik - angleški
    COBISS.SI-ID - 28890329
    DOI

Vnos na polico