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  • TREK-1 channels regulate pressure sensitivity and calcium signaling in trabecular meshwork cells
    Yarishkin, Oleg ...
    Mechanotransduction by the trabecular meshwork (TM) is an essential component of intraocular pressure regulation in the vertebrate eye. This process is compromised in glaucoma but is poorly ... understood. In this study, we identify transient receptor potential vanilloid isoform 4 (TRPV4) and TWIK-related potassium channel-1 (TREK-1) as key molecular determinants of TM membrane potential, pressure sensitivity, calcium homeostasis, and transcellular permeability. We show that resting membrane potential in human TM cells is unaffected by "classical" inhibitors of voltage-activated, calcium-activated, and inwardly rectifying potassium channels but is depolarized by blockers of tandem-pore K+ channels. Using gene profiling, we reveal the presence of TREK-1, TASK-1, TWIK-2, and THIK transcripts in TM cells. Pressure stimuli, arachidonic acid, and TREK-1 activators hyperpolarize these cells, effects that are antagonized by quinine, amlodipine, spadin, and short-hairpin RNA-mediated knockdown of TREK-1 but not TASK-1. Activation and inhibition of TREK-1 modulates [Ca2+]TM and lowers the impedance of cell monolayers. Together, these results suggest that tensile homeostasis in the TM may be regulated by balanced, pressure-dependent activation of TRPV4 and TREK-1 mechanotransducers.
    Vir: The Journal of general physiology. - ISSN 0022-1295 (Vol. 150, no. 12, Dec. 2018, str. 1660-1675)
    Vrsta gradiva - članek, sestavni del ; neleposlovje za odrasle
    Leto - 2018
    Jezik - angleški
    COBISS.SI-ID - 34093017

    Povezava(-e):

    http://jgp.rupress.org/content/150/12/1660.long
    DOI

vir: The Journal of general physiology. - ISSN 0022-1295 (Vol. 150, no. 12, Dec. 2018, str. 1660-1675)
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