VSE knjižnice (vzajemna bibliografsko-kataložna baza podatkov COBIB.SI)
  • Zinc-phosphate nanoparticles with reversibly attached TNF-▫$alfa$▫ analogs
    Hribar, Gorazd, 1980- ...
    The authors: intention was to prepare nanometer-sized zinc-phosphate nanoparticles that would be capable of binding histidine-rich TNF-a analogs onto their surface via a coordinative bond. ... Zinc-phosphate nanoparticles with a size of around 60 nm were prepared by a wet precipitation method and characterized using SEM, EDX, XRD, and DLS. First, BSA was bound as a testing protein, afterward two TNF-a analogs with decreased activity were bound to thedescribed nanoparticles. The efficiency of binding and the existence of coordinative bond were confirmed with SDS-PAGE analysis. During binding, particle storage, and release experiments, the prepared TNF-a analogs retainedtheir biological activity-hence the epitopes necessary for formation of antibodies stayed intact. The particle size did not change within a period of 2 weeks. No significant agglomeration was observed, the particles could be quickly dispersed in ultrasound. The present nanoparticles and the general approach of coordinative binding are widely applicable for natural and engineered histidine-rich proteins. The nanoparticles bearing appropriate TNF-a analogs could also be potentially used for active immunotherapy to tackle the chronic inflammatory diseases associated with pathogenically elevated levels of TNF-a.
    Vir: Journal of nanoparticle research. - ISSN 1388-0764 (Vol. 13, no. 7, 2011, str. 3019-3032)
    Vrsta gradiva - članek, sestavni del
    Leto - 2011
    Jezik - angleški
    COBISS.SI-ID - 4607770
    DOI

Vnos na polico