In this paper, the content of labile hot water soluble organic matter (HWOC) was used as an indicator for assessing the condition and degradation of the soil. The results of the research, which ...included different land uses, crop rotations, plant species, cover crops, depth and time of sampling, were compared in order to assess their effects on HWOC dynamic. It was found that the level of labile organic matter in the soil ranges from 1–5% of stable organic matter and samples originating from non-agricultural land have significantly higher values compared to those found on the arable land. Based on the obtained results, it was determined that with the increase of labile OM in the soil depending on the mineralization rate, the dominant carbon source for microorganisms becomes the labile fraction and in such conditions there will be no changes in the stable OM fraction and its decrease. If the amount of labile OM is lower, carbon consumption occurs, which leads to a decrease in the content of the stable fraction (humus), that in the long run results in soil quality decrease. Our research has shown that there are large differences in the obtained values, but the land use plays a dominant role in the dynamics of labile OM, while the application of manure and conservation tillage contribute to the preservation of HWOC on arable land. The presented data can contribute to a better understanding of the role of labile matter in the soil and its relationship with other soil properties, which can be used for improvement of crop cultivation technology and yield increase.
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