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Uporaba molekularnih metod za določanje fitopatogenih bakterij na primeru Erwinia amylovora (Burrill) Winslow et al. = The use of molecular biology methods for detection of phytopathogenic bacteria: the study case of Erwinia amylovora (Burrill) Winslow et al.
Demšar, Tina, 1973- ...
Fireblight, caused by bacteria Erwinia amylovora (Burrill) Winslow et al., is a quarantine disease on fruit trees and ornamental plants recorded in most European countries. The fact that fireblight ...is present in all countries neighbouring Slovenia represents a very high possibility of it's introduction to Slovenia, which is a serious treat to Slovenian fruit growing, especially production of pears and apples. A testing procedure reecomended by EPPO (European Plant Protection Organization) includes: isolation of bacteria by plafing on semiselective and non-selective media, serological meth ods (agglutination and immunofluorescence), biochemical tests and pathogenicity test on immature pear fruits. As an addition to all these laboratory tests we have introduced a molecular biology based method (PCR), which both confirms the Erwinia amylavora (Ea) in a bacterial culture, and enables to distinguish Ea from other bacteria such as Pseudomonas syringae pv. syringae, a causal agent of a disease similar to fireblight regarding the symptoms, and from other frequently present bacteria on fruit trees. The results of PCR testing with the use of different primers, which amplify bacterial DNA is presented.
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